Composite

Part:BBa_K4211054:Design

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-10-04)


LightOn-CenA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene of interest (GOI) is replaced with CenA by BNUZH-China iGEM team 2022 to cut internal β-1,4-glucosidic bonds.

Source

It was designed by the BNUZH-China iGEM team 2022 based on the article in Nature Methods: Spatiotemporal control of gene expression by a light-switchable transgene system.

References

Wang, X., Chen, X. & Yang, Y. Spatiotemporal control of gene expression by a light-switchable transgene system. Nat Methods 9, 266-269 (2012). https://doi.org:10.1038/nmeth.1892